Journal: bioRxiv

Article Title: HAND1 knockdown disrupts trophoblast global gene expression

doi: 10.1101/2022.11.01.514704

Figure Lengend Snippet: A . In BeWo cells, HAND1 mRNA expression was reduced by 61% compared to Allstar negative control treatment after 96 h. D . In HPMVECs, HAND1 mRNA expression was reduced by 69% compared to Allstar negative control treatment after 96 h. Data are median ± interquartile range. Statistical significance was determined using Mann-Whitney test. n = 3 to 9 passages

Article Snippet: Human Placenta Microvascular Endothelial Cells (HPMVECs) were cultured in T75 flasks pre-treated with attachment factor (Cell Applications Inc.) at 37°C, 5% CO 2 in EGM-2 media (Lonza, Allendale, NJ).

Techniques: Expressing, Negative Control, MANN-WHITNEY

Journal: bioRxiv

Article Title: HAND1 knockdown disrupts trophoblast global gene expression

doi: 10.1101/2022.11.01.514704

Figure Lengend Snippet: A . mRNA expression of GADD45G was shown to be reduced in HAND1 siRNA treated Human Placenta Microvascular Endothelial cells (HPMVECs) when compared to Allstar negative control treated cells. B . Expression of NPPB was increased in in HAND1 siRNA treated HPMVECs when compared to Allstar negative control treated cells. Data are median ± interquartile range. Statistical significance was determined using Mann-Whitney test. n = 3 passages

Article Snippet: Human Placenta Microvascular Endothelial Cells (HPMVECs) were cultured in T75 flasks pre-treated with attachment factor (Cell Applications Inc.) at 37°C, 5% CO 2 in EGM-2 media (Lonza, Allendale, NJ).

Techniques: Expressing, Negative Control, MANN-WHITNEY

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Synergistic Cytotoxicity of Extracts of Chaga Mushroom and Microalgae against Mammalian Cancer Cells In Vitro

doi: 10.1155/2024/7944378

Figure Lengend Snippet: Effect of supercritical I. obliquus extracts on cancer and normal mammalian cells. Cancer cell and nonmalignant normal cell lines were treated with various doses of two supercritical extracts (see ) of I. obliquus , and cell viability was determined after 24 hr. (a) Comparison between human breast epithelial adenocarcinoma (MCF-7) and human breast epithelial cell (MCF-10A) cells. (b) Comparison between human osteosarcoma (HOS) and canine osteoplast (Cnob) cells. (c) Comparison between canine osteosarcoma (D-17) and Cnob cells. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001.

Article Snippet: DH-82, HOS, and D-17 cells were cultured in EMEM (Sigma–Aldrich, Oakville, ON, Canada), MCF-7 and HepG2 in DMEM (Gibco), CnOb in CnOb basal medium supplemented with growth medium (cell applications), MCF-10A cultured in basal medium supplemented with growth medium, and THLE-3 in BEGM with supplements (Lonza Bioscience, Burlington, ON, Canada), as recommended.

Techniques: Comparison

Journal: bioRxiv

Article Title: Hierarchically Vascularized and Implantable Tissue Constructs created through Angiogenesis from Tissue-Engineered Vascular Grafts

doi: 10.1101/2024.04.29.591796

Figure Lengend Snippet: The process of fabricating engineered tissue. a-i) Tubular PCL scaffolds were fabricated via electrospinning and a-ii) machined to create macropores to enable angiogenesis. a-iii) The presence of the pores was confirmed through visual inspection and SEM imaging (scale bar: 100 µm). b-i and ii) A GelMA hydrogel layer (with or without SMCs) was cast and cured around the electrospun scaffold, and b-iii and iv) the luminal surface was seeded with endothelial cells to enable the formation of an endothelium and sprouting angiogenesis. b-v) Photograph of a TEGV with macropores. c) Creating a large, vascularized tissue by using two vascular grafts is also possible by c-i) casting a GelMA mixture around two electrospun tubes, c-ii) cross-linking the GelMA layer, and c-iii) Seeding ECs in the lumens of the PCL tubes. c-iv) This has the potential to generate large, vascularized tissues. c-v) Photograph of a large construct incorporating two TEVGs.

Article Snippet: Human umbilical vein ECs (Lonza, USA) and GFP expressing human umbilical vein ECs (Angio-Proteomie, USA) were cultured in EGM-2 medium (Lonza, USA) and endothelial growth medium ECs (Angio-Proteomie, USA), respectively, with 1% v/v antibiotic-antimycotic solution (Gibco, USA).

Techniques: Imaging, Construct

Journal: bioRxiv

Article Title: Hierarchically Vascularized and Implantable Tissue Constructs created through Angiogenesis from Tissue-Engineered Vascular Grafts

doi: 10.1101/2024.04.29.591796

Figure Lengend Snippet: Endothelialization of the vascular grafts. a) A confocal image showing circumferential coverage of ECs on the lumen surface of the vascular graft after 7 days of culture, red: actin filaments stain (ActinRed™ 555), blue: nuclei stain (DAPI). b) Confocal images of ECs on the surface of the graft lumen at different time points showing the increase of endothelial coverage and proximity of the cells; scale bar = 50 μm. c) Quantitative analysis of EC coverage by area of the lumen over time. Error bars represent the standard deviation (n = 12; ***: p < 0.001, one-way ANOVA).

Article Snippet: Human umbilical vein ECs (Lonza, USA) and GFP expressing human umbilical vein ECs (Angio-Proteomie, USA) were cultured in EGM-2 medium (Lonza, USA) and endothelial growth medium ECs (Angio-Proteomie, USA), respectively, with 1% v/v antibiotic-antimycotic solution (Gibco, USA).

Techniques: Staining, Standard Deviation